Creating tools to engineer biology: The Onyx editing method
Filling the gap between the potential of biotechnology and existing genomic engineering capabilities requires a novel approach to be able to engineer cells en masse, coupled with a feasible way to monitor phenotypic outcomes. The Inscripta® Onyx® solution uses the patented CRISPR-enabled trackable genome engineering (CREATE) technology . The CREATE method relies on 3 essential components expressed from a single plasmid: the gRNA, the editing cassette, and a barcode sequence to identify the edit. The link between the genomic edit and the barcode allows identification of the engineered cells by simply sequencing the barcoded region of the plasmid and tracking of tens of thousands of edited cells within a population.