Target type: Pathway, genome-wide
Purpose: Localization of enzymes plays a key role in their biological function. Disrupting or “re-wiring” localization within the cell can cause significant biological changes which may be beneficial to desired phenotypes.
Example of libraries: Modify the secretory machinery of the cell by changing the localization of key enzymes to different cellular compartments. For all proteins known to be involved in secretion, replace any endogenous localization signals with an NLS (sequester in the nucleus) and Golgi localization signal.