Onyx® iterative editing.


Design. Discover. Repeat.

Iterative Genome Engineering allows you rapidly move through the Design-Generate-Test-Learn (DGTL) cycle to accelerate the discovery process. After screening your Onyx Cell Libraries and identifying the variants you want to take forward, you can design new edit libraries to generate additional diversity. To do that, the editing plasmids are cured from the engineered cells, which can be then transformed with new editing reagents. Update and import the new genome in just a few minutes using our software and proceed to design your next Genome Engineering Experiment.
Design-generate-test-learn cycles accelerated with Onyx Iterative Editing

Onyx makes iterative genome engineering easy.

Inscripta’s Iterative Genome Engineering protocols and kits enable easy workflow integration, with step-by-step validated instructions on how to prepare your strains for the next genome engineering cycle. Now you can get to the next round of editing in less than a week.
E. coli

We offer an Onyx® E. coli Curing Kit enabling instrument curing of up to 24 variants and supporting documents describing the curing and preparation for Iterative Genome Engineering. 

S. cerevisiae

For S. cerevisiae, we provide a validated, manual plasmid curing protocol (up to 24 variants), as well as protocols describing the preparation of strains for Iterative Genome Engineering. 


See what iterative genome engineering can do for you.

Whether you are working on engineering a more efficient enzyme or trying to improve productivity of your strain, in just a few genome editing cycles you can achieve unprecedented results. See how you can rapidly achieve significant strain improvement with the Onyx Iterative Genome Engineering workflow.

Eric Abbate — Director of Analytical Biochemistry, Applications Development, Inscripta

Onyx E. coli Curing Kit for iterative editing.

Feature Specification


E. coli


Inscripta standard and customer strains

Pooling capacity

2 to 24 variants

Curing efficiency


Instrument time

43 hours


Generate any library at the push of a button.

The Onyx gives you the flexibility and scalability to develop virtually limitless libraries. Create insertions, deletions, substitutions, or full saturation mutagenesis, in a single gene, a pathway, or the whole genome.