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Massively Parallel CRISPR Genome Editing in S. cerevisiae

In this webinar, we will discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise genomic edits have been created and tested to accelerate forward engineering and genomic discovery.

Efficient and Rapid Discovery of Novel Temperature Sensitive Alleles with the Onyx ® Digital Genome Engineering Platform
Temperature sensitive (TS) alleles are mutations in essential genes that allow for growth at permissive temperatures and restrict or reduce...
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Accelerating strain development process for CDMO and CRO companies
Manufacturing of proteins, mRNA or small molecules requires comprehensive strain engineering approaches to increase yield, productivity, and robustness of the...
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Improving protein expression in E. coli B‑strains using rapid genome engineering
E. coli B‑strains, such as BL21, and their derivatives are often used for protein expression due to their optimized amino...
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