Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform

In this webinar, we discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise, genomic edits have been created and tested to accelerate forward engineering and genomic discovery.