The MAD7 nuclease: made for discovery.

Democratizing genome engineering

Inspired by the biological diversity found on the island of Madagascar, the MADzyme nucleases have improved features over commonly used CRISPR-Cas9 nucleases, such as different PAM recognition sequences and cut efficiencies, fewer off-target effects, reduced sizes and differing enzyme kinetics. 

We developed and publicly released the MAD7 nuclease, the first of the MADzyme nucleases, to promote widespread adoption of CRISPR tools in academic and commercial settings. The MAD7 nuclease has already been shown to be effective in microbial, plant, fish and mammalian systems. Go ahead and try it, the MAD7 nuclease is royalty-free for both academic and commercial research and development use.

MAD7 nuclease structural model

The MAD7 nuclease in mammalian cells

In addition to robust performance in prokaryotic and eukaryotic microbial systems, the MAD7 nuclease has demonstrated editing activity in mammalian cells. 

For example, initial experiments in HEK293T cells show that the MAD7 nuclease can be expressed as an active protein in mammalian cells, and when combined with synthetic guide RNAs, can edit different genes at multiple loci.


Is the MAD7 nuclease really free?

For academic and commercial R&D, yes! Inscripta provides the DNA sequence of the E. coli codon-optimized MAD7 nuclease without reach-through royalty rights. Royalties are ONLY attached to the use of the MAD7 nuclease if a commercial manufacturing process uses the MAD7 nuclease on an ongoing basis or a product physically contains the MAD7 nuclease. A license is provided when the sequence is downloaded, and if requested, Inscripta can provide a hard copy license for free use of the MAD7 nuclease in R&D.