RESOURCES AND SUPPORT

Powerful support for scientists.

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Videos

March 26, 2021
Onyx Customer Spotlight — Sestina Bio [5:22]
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March 10, 2021
Onyx Platform Launch Video [0:40]
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January 11, 2021
How the Onyx Digital Genome Engineering Platform Works [5:24]
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December 30, 2020
Introduction to InscriptaDesigner Software [4:59]
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December 30, 2020
A Quick Guide to the InscriptaResolver Tools [4:57]
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November 19, 2020
Onyx Genome Engineering Platform Introduction [2:40]
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March 26, 2021
Onyx Customer Spotlight — Sestina Bio [5:22]
Watch Video
November 19, 2020
Onyx Genome Engineering Platform Introduction [2:40]
Watch Video
March 10, 2021
Onyx Platform Launch Video [0:40]
Watch Video
January 11, 2021
How the Onyx Digital Genome Engineering Platform Works [5:24]
Watch Video
December 30, 2020
Introduction to InscriptaDesigner Software [4:59]
Watch Video
December 30, 2020
A Quick Guide to the InscriptaResolver Tools [4:57]
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Presentations & webinars

Webinar coming soon
May 25, 2021
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Register Now
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Date: May 25, 2021
Speaker: Bryan Leland
Abstract: In this webinar, we will discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Register Now
2021 April webinar image
April 27, 2021
Proteins, Pathways and Genomes: Innovations in Forward Engineering
View More
Proteins, Pathways and Genomes: Innovations in Forward Engineering
Date: April 27, 2021
Speaker: Eric Abbate
Abstract: Lysine, predominately used as an animal feed supplement, is a multi-billion-dollar industry however supply is limited due to the cost of current production methods. This webinar will share case studies how Inscripta leveraged its new CRISPR-based technology to design, generate, and screen libraries totaling > 200,000 single edits to improve lysine production in E. coli. Dozens of beneficial edits across the entire genome were discovered to accelerate the Design-Generate-Test-Learn cycle and increase the lysine production by 14,000-fold — ushering in the next era of genome editing. 
View More
Tyson
March 30, 2021
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
View More
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
Date: March 30, 2021
Speaker: Tyson Shepherd
Abstract: Since its introduction, CRISPR has become a core genome editing tool that has transformed biological research. First-generation CRISPR technologies were limited in scalability, accessibility, edit variety, and ease of use, restricting their potential. Inscripta’s Onyx platform addresses these limitations by combining easy-to-use, intuitive software with a push-button automated benchtop device, enabling high-efficiency, massively-parallel, precision-engineered edits to Saccharomyces cerevisiae and Escherichia coli genomes. In this webinar , Tyson Shepherd will present applications in E. coli that leverage this platform. He will show how a library totaling more than 25,000 different designs and a separate library of 900 designs yielded new biochemical insights underpinning tolerance to a panel of growth-inhibitory compounds. These applications demonstrate the power of the Onyx platform to usher in a new era of genome editing.
Watch Video
High
January 26, 2021
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
View More
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
Date: January 26, 2021
Speaker: Liselot Dewachter and Laura Klitten
Abstract: In this study, we used the high-throughput CRISPR-based Onyx platform to perform saturation mutagenesis on four different essential genes involved in cell envelope synthesis in E. coli. 22,790 edits were designed. We used these saturation mutagenesis libraries to probe the essentiality of all different residues. Edits that could not be introduced suggests those residues are essential for protein function. We identified known essential amino acids (i.e. catalytic residues and residues involved in substrate binding), thereby validating our experimental approach. Several residues that were previously not known to be essential were identified. We expect our results to offer vastly improved insights into protein function, help fight against antibiotic resistance and aid structure-based drug design targeted against these essential proteins.
Watch Video
Leland
October 20, 2020
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
View More
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
Date: October 20, 2020
Speaker: Bryan Leland
Abstract: In this webinar, we discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise, genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Watch Video
Nandini
September 30, 2020
Massively parallel microbial genome engineering using the Onyx Platform
View More
Massively parallel microbial genome engineering using the Onyx Platform
Date: September 30, 2020
Speaker: Nandini Krishnamurthy
Abstract: While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, and ease of use. Inscripta’s Onyx platform addresses these limitations by offering push-button, high efficiency, massively parallel, and precise delivery of diverse edit types across the genomes of S. cerevisiae and E. coli. The platform simplifies previously complex CRISPR strain engineering workflows by offering an end-to-end solution from design through engineered strain library and analytics, including software, reagents and a benchtop instrument. Join us to learn more about this platform and see examples of engineering genomes at unprecedented scale end efficiency.
Watch Video
Part 2
September 1, 2020
Rapid Forward Engineering of Biological Systems: Part 2 Combinatorial Optimization
View More
Rapid Forward Engineering of Biological Systems: Part 2 Combinatorial Optimization
Date: September 1, 2020
Speaker: Richard Fox
Abstract: Many of the governing rules of biology are still not known nor well understood. Thus, forward engineering of biological systems will continue to rely on empirical methods. A central challenge in forward engineering is to find genetic interventions that lead to improved function. Combinatorial optimization (fueled by large-scale diversity generation) takes ideas that show promise and recombines them in novel configurations to leverage the principles of evolutionary optimization. This optimization process is further accelerated through strategies that incorporate machine learning. Such models are interrogated to efficiently guide new library designs. Strategies and tools to effectively apply the principles of diversity generation (and combinatorial optimization) have been developed over the last two decades and are now routinely used to rapidly engineer biological systems.
Watch Video
Part 1
August 25, 2020
Rapid Forward Engineering of Biological Systems: Part 1 Diversity Generation
View More
Rapid Forward Engineering of Biological Systems: Part 1 Diversity Generation
Date: August 25, 2020
Speaker: Richard Fox
Abstract: Significant potential exists to harness the power of biology to better humanity and the environment, however, substantial challenges remain. Forward engineering of biological systems will continue to rely on empirical methods. Diversity generation is one of two key principles needed to reach desired outcomes in a rapid, efficient and cost-effective manner. Sequence space is vast. A central challenge in forward engineering is to find genetic interventions that lead to improved function. A large-scale diversity generation approach, where many thousands of ideas are rapidly tested, has proved to be crucial to addressing this challenge. Strategies and tools to effectively apply the principles of diversity generation (and combinatorial optimization) have been developed over the last two decades and are now routinely used to rapidly engineer biological systems.
Watch Video
Stephen
May 14, 2020
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
View More
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
Date: May 14, 2020
Speaker: Stephen Federowicz
Abstract: Massively parallel genome engineering enables rapid and simultaneous evaluation of genotype-phenotype relationships at a genomic scale. With the Inscripta Onyx Platform, we replaced every promoter in the E. coli genome with one of five synthetic constitutive promoters across an expression ladder. Additionally, we generated two versions of a genome scale knockout library by inserting three premature stop codons in every gene at two different positions near the 5’ end. We then pooled these libraries for a total of 23,576 genomic edits, and under strong selective pressure quantified shifts in the edited populations to determine relative strain performance. We readily identify thousands of genotype-phenotype interactions that confirm known mechanisms and reveal large sets of novel interactions – demonstrating the power of high-efficiency automated genome engineering.
Watch Video
High
January 26, 2021
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
View More
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
Date: January 26, 2021
Speaker: Liselot Dewachter and Laura Klitten
Abstract: In this study, we used the high-throughput CRISPR-based Onyx platform to perform saturation mutagenesis on four different essential genes involved in cell envelope synthesis in E. coli. 22,790 edits were designed. We used these saturation mutagenesis libraries to probe the essentiality of all different residues. Edits that could not be introduced suggests those residues are essential for protein function. We identified known essential amino acids (i.e. catalytic residues and residues involved in substrate binding), thereby validating our experimental approach. Several residues that were previously not known to be essential were identified. We expect our results to offer vastly improved insights into protein function, help fight against antibiotic resistance and aid structure-based drug design targeted against these essential proteins.
Watch Video
Webinar coming soon
May 25, 2021
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Register Now
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Date: May 25, 2021
Speaker: Bryan Leland
Abstract: In this webinar, we will discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Register Now
2021 April webinar image
April 27, 2021
Proteins, Pathways and Genomes: Innovations in Forward Engineering
View More
Proteins, Pathways and Genomes: Innovations in Forward Engineering
Date: April 27, 2021
Speaker: Eric Abbate
Abstract: Lysine, predominately used as an animal feed supplement, is a multi-billion-dollar industry however supply is limited due to the cost of current production methods. This webinar will share case studies how Inscripta leveraged its new CRISPR-based technology to design, generate, and screen libraries totaling > 200,000 single edits to improve lysine production in E. coli. Dozens of beneficial edits across the entire genome were discovered to accelerate the Design-Generate-Test-Learn cycle and increase the lysine production by 14,000-fold — ushering in the next era of genome editing. 
View More
Tyson
March 30, 2021
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
View More
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
Date: March 30, 2021
Speaker: Tyson Shepherd
Abstract: Since its introduction, CRISPR has become a core genome editing tool that has transformed biological research. First-generation CRISPR technologies were limited in scalability, accessibility, edit variety, and ease of use, restricting their potential. Inscripta’s Onyx platform addresses these limitations by combining easy-to-use, intuitive software with a push-button automated benchtop device, enabling high-efficiency, massively-parallel, precision-engineered edits to Saccharomyces cerevisiae and Escherichia coli genomes. In this webinar , Tyson Shepherd will present applications in E. coli that leverage this platform. He will show how a library totaling more than 25,000 different designs and a separate library of 900 designs yielded new biochemical insights underpinning tolerance to a panel of growth-inhibitory compounds. These applications demonstrate the power of the Onyx platform to usher in a new era of genome editing.
Watch Video
Leland
October 20, 2020
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
View More
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
Date: October 20, 2020
Speaker: Bryan Leland
Abstract: In this webinar, we discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise, genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Watch Video
Nandini
September 30, 2020
Massively parallel microbial genome engineering using the Onyx Platform
View More
Massively parallel microbial genome engineering using the Onyx Platform
Date: September 30, 2020
Speaker: Nandini Krishnamurthy
Abstract: While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, and ease of use. Inscripta’s Onyx platform addresses these limitations by offering push-button, high efficiency, massively parallel, and precise delivery of diverse edit types across the genomes of S. cerevisiae and E. coli. The platform simplifies previously complex CRISPR strain engineering workflows by offering an end-to-end solution from design through engineered strain library and analytics, including software, reagents and a benchtop instrument. Join us to learn more about this platform and see examples of engineering genomes at unprecedented scale end efficiency.
Watch Video
Part 2
September 1, 2020
Rapid Forward Engineering of Biological Systems: Part 2 Combinatorial Optimization
View More
Rapid Forward Engineering of Biological Systems: Part 2 Combinatorial Optimization
Date: September 1, 2020
Speaker: Richard Fox
Abstract: Many of the governing rules of biology are still not known nor well understood. Thus, forward engineering of biological systems will continue to rely on empirical methods. A central challenge in forward engineering is to find genetic interventions that lead to improved function. Combinatorial optimization (fueled by large-scale diversity generation) takes ideas that show promise and recombines them in novel configurations to leverage the principles of evolutionary optimization. This optimization process is further accelerated through strategies that incorporate machine learning. Such models are interrogated to efficiently guide new library designs. Strategies and tools to effectively apply the principles of diversity generation (and combinatorial optimization) have been developed over the last two decades and are now routinely used to rapidly engineer biological systems.
Watch Video
Part 1
August 25, 2020
Rapid Forward Engineering of Biological Systems: Part 1 Diversity Generation
View More
Rapid Forward Engineering of Biological Systems: Part 1 Diversity Generation
Date: August 25, 2020
Speaker: Richard Fox
Abstract: Significant potential exists to harness the power of biology to better humanity and the environment, however, substantial challenges remain. Forward engineering of biological systems will continue to rely on empirical methods. Diversity generation is one of two key principles needed to reach desired outcomes in a rapid, efficient and cost-effective manner. Sequence space is vast. A central challenge in forward engineering is to find genetic interventions that lead to improved function. A large-scale diversity generation approach, where many thousands of ideas are rapidly tested, has proved to be crucial to addressing this challenge. Strategies and tools to effectively apply the principles of diversity generation (and combinatorial optimization) have been developed over the last two decades and are now routinely used to rapidly engineer biological systems.
Watch Video
Stephen
May 14, 2020
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
View More
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
Date: May 14, 2020
Speaker: Stephen Federowicz
Abstract: Massively parallel genome engineering enables rapid and simultaneous evaluation of genotype-phenotype relationships at a genomic scale. With the Inscripta Onyx Platform, we replaced every promoter in the E. coli genome with one of five synthetic constitutive promoters across an expression ladder. Additionally, we generated two versions of a genome scale knockout library by inserting three premature stop codons in every gene at two different positions near the 5’ end. We then pooled these libraries for a total of 23,576 genomic edits, and under strong selective pressure quantified shifts in the edited populations to determine relative strain performance. We readily identify thousands of genotype-phenotype interactions that confirm known mechanisms and reveal large sets of novel interactions – demonstrating the power of high-efficiency automated genome engineering.
Watch Video
2021 April webinar image
April 27, 2021
Proteins, Pathways and Genomes: Innovations in Forward Engineering
View More
Proteins, Pathways and Genomes: Innovations in Forward Engineering
Date: April 27, 2021
Speaker: Eric Abbate
Abstract: Lysine, predominately used as an animal feed supplement, is a multi-billion-dollar industry however supply is limited due to the cost of current production methods. This webinar will share case studies how Inscripta leveraged its new CRISPR-based technology to design, generate, and screen libraries totaling > 200,000 single edits to improve lysine production in E. coli. Dozens of beneficial edits across the entire genome were discovered to accelerate the Design-Generate-Test-Learn cycle and increase the lysine production by 14,000-fold — ushering in the next era of genome editing. 
View More
Tyson
March 30, 2021
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
View More
A New Era in Forward Engineering of Proteins, Pathways and Genomes using the Onyx Platform
Date: March 30, 2021
Speaker: Tyson Shepherd
Abstract: Since its introduction, CRISPR has become a core genome editing tool that has transformed biological research. First-generation CRISPR technologies were limited in scalability, accessibility, edit variety, and ease of use, restricting their potential. Inscripta’s Onyx platform addresses these limitations by combining easy-to-use, intuitive software with a push-button automated benchtop device, enabling high-efficiency, massively-parallel, precision-engineered edits to Saccharomyces cerevisiae and Escherichia coli genomes. In this webinar , Tyson Shepherd will present applications in E. coli that leverage this platform. He will show how a library totaling more than 25,000 different designs and a separate library of 900 designs yielded new biochemical insights underpinning tolerance to a panel of growth-inhibitory compounds. These applications demonstrate the power of the Onyx platform to usher in a new era of genome editing.
Watch Video
High
January 26, 2021
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
View More
High-throughput CRISPR editing using the Onyx platform identifies essential residues in proteins
Date: January 26, 2021
Speaker: Liselot Dewachter and Laura Klitten
Abstract: In this study, we used the high-throughput CRISPR-based Onyx platform to perform saturation mutagenesis on four different essential genes involved in cell envelope synthesis in E. coli. 22,790 edits were designed. We used these saturation mutagenesis libraries to probe the essentiality of all different residues. Edits that could not be introduced suggests those residues are essential for protein function. We identified known essential amino acids (i.e. catalytic residues and residues involved in substrate binding), thereby validating our experimental approach. Several residues that were previously not known to be essential were identified. We expect our results to offer vastly improved insights into protein function, help fight against antibiotic resistance and aid structure-based drug design targeted against these essential proteins.
Watch Video
Nandini
September 30, 2020
Massively parallel microbial genome engineering using the Onyx Platform
View More
Massively parallel microbial genome engineering using the Onyx Platform
Date: September 30, 2020
Speaker: Nandini Krishnamurthy
Abstract: While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, and ease of use. Inscripta’s Onyx platform addresses these limitations by offering push-button, high efficiency, massively parallel, and precise delivery of diverse edit types across the genomes of S. cerevisiae and E. coli. The platform simplifies previously complex CRISPR strain engineering workflows by offering an end-to-end solution from design through engineered strain library and analytics, including software, reagents and a benchtop instrument. Join us to learn more about this platform and see examples of engineering genomes at unprecedented scale end efficiency.
Watch Video
Stephen
May 14, 2020
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
View More
Massively parallel genome engineering followed by pooled growth selections for rapid target discovery in microbes
Date: May 14, 2020
Speaker: Stephen Federowicz
Abstract: Massively parallel genome engineering enables rapid and simultaneous evaluation of genotype-phenotype relationships at a genomic scale. With the Inscripta Onyx Platform, we replaced every promoter in the E. coli genome with one of five synthetic constitutive promoters across an expression ladder. Additionally, we generated two versions of a genome scale knockout library by inserting three premature stop codons in every gene at two different positions near the 5’ end. We then pooled these libraries for a total of 23,576 genomic edits, and under strong selective pressure quantified shifts in the edited populations to determine relative strain performance. We readily identify thousands of genotype-phenotype interactions that confirm known mechanisms and reveal large sets of novel interactions – demonstrating the power of high-efficiency automated genome engineering.
Watch Video
Webinar coming soon
May 25, 2021
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Register Now
Coming Soon: Massively Parallel CRISPR Genome Editing in S. cerevisiae
Date: May 25, 2021
Speaker: Bryan Leland
Abstract: In this webinar, we will discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Register Now
Leland
October 20, 2020
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
View More
Massively parallel CRISPR genome editing in S. cerevisiae using the Onyx Platform
Date: October 20, 2020
Speaker: Bryan Leland
Abstract: In this webinar, we discuss our work using the Onyx platform to perform genome-wide engineering of S. cerevisiae for various applications including target discovery to increase glycerol utilization, strain optimization, and forward engineering. These applications are enabled by Onyx’s ability to deliver diverse edit types that regulate gene function and expression beyond simple gene knock-outs. In total, dozens of libraries of engineered cells, containing over 100,000 precise, genomic edits have been created and tested to accelerate forward engineering and genomic discovery.
Watch Video
Nandini
September 30, 2020
Massively parallel microbial genome engineering using the Onyx Platform
View More
Massively parallel microbial genome engineering using the Onyx Platform
Date: September 30, 2020
Speaker: Nandini Krishnamurthy
Abstract: While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, and ease of use. Inscripta’s Onyx platform addresses these limitations by offering push-button, high efficiency, massively parallel, and precise delivery of diverse edit types across the genomes of S. cerevisiae and E. coli. The platform simplifies previously complex CRISPR strain engineering workflows by offering an end-to-end solution from design through engineered strain library and analytics, including software, reagents and a benchtop instrument. Join us to learn more about this platform and see examples of engineering genomes at unprecedented scale end efficiency.
Watch Video
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Faq

FAQ

Get answers to commonly asked questions in our FAQ.

FAQ
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Service & Support

Inscripta provides dedicated support through every step of your genome engineering experiments. Our Field Applications, Field Service, and Technical Support teams assist you with in-depth project consultation, customized applications design, comprehensive instrument installation, training and maintenance, and detailed data analysis and interpretation. When you are ready to begin, you can log in to the Inscripta Engineering Portal to upload genomes, design experiments, place orders, monitor instrument runs, and analyze results.

For help with our software, consumables, assays, and the Onyx Instrument, contact us at support@​inscripta.​com.