Onyx® Genome Engineering kits


Meet your customized genome engineering kit

Onyx Genome Engineering Kits are customized for your experiment and leverage our patented Onyx chemistry to enable precise editing of the entire genome, and accurate tracking of the edited cell population. Design and order your kit from our portal and three weeks later receive your ready-to-use library of up to 10,000 Design DNAs and all consumables and reagents ready to load onto the Onyx Instrument.


Onyx chemistry. Thousands of edits in a single tube.

In a traditional CRISPR experiment, one edit is performed per well and multiplexing is not an option — one gRNA and the corresponding homology arm are added per well to ensure that a cell will only receive one gRNA and donor DNA template. By linking the gRNA with the donor template, it’s now possible to multiplex up to 10,000 edits in a single tube.
One Design DNA per cell.

gRNA, donor DNA template and barcode are linked together in a single unique Design DNA. Each transformed cell receives only one Design DNA, generating one edit per cell.

One cartridge per experiment.

One ready-to-use cartridge contains up to 10,000 Design DNA, enabling massively parallel cell library generation in a single Onyx engineering run.

One tube. Thousands of Onyx edits.

The Onyx cell library obtained at the end of the run contains millions of cells with thousands of complete and intended edits.

Onyx chemistry infographic

Onyx chemistry. Innovative mechanism.

The Design DNA contains not only the gRNA-Donor DNA-Barcode but also all other components required to perform the editing in vivo such as the MAD7 and the repair proteins. Inscripta’s proprietary high-efficiency editing process is entirely controlled by the Onyx Instrument with almost no human intervention.
Innovative mechanism5

Genome engineering kit specifications

Feature Specification


E. coli; S. cerevisiae


Inscripta standard and customer strains


1 edit per cell

Edit Types

Insertions, deletions and substitutions, up to 63 bp

# of designs / experiment

100−10,000 E. coli; 100−6,000 S. cerevisiae

% Edited Cells

Typically, between 15% and 75%, depending on library

Custom Reagent Order Fulfillment

3 weeks

Instrument Time

2 to 4 days, depending on strain


Plasmid curing

Following a Genome Engineering Experiment, cells may require the removal of the editing plasmids. This step, referred to as curing, is required to perform subsequent rounds of Iterative Genome Engineering or for eliminating the plasmids to prepare cells for downstream applications. For S. cerevisiae strains, plasmid curing is performed simply through a series of growth steps in the absence of selective pressure (see Onyx Plasmid Curing and Iterative Genome Engineering Handbook — S. cerevisiae for protocol). For E. coli strains, we have developed an Onyx E.coli Curing Kit enabling instrument curing of up to 24 variants in one run.

Onyx E. coli Curing Kit specifications

Feature Specification


E. coli


Inscripta standard and customer strains

Pooling capacity

2 to 24 variants

Curing efficiency


Instrument time

43 hours


Want to use your own strain?

Inscripta’s Genome Engineering Kits are designed for compatibility with widely used and validated strains, but researchers have the option to use their own strain as well. We offer the Onyx Strain Assessment Kit for your E. coli or S. cerevisiae strain of choice to determine whether your strain is compatible with the Onyx Platform.

Evaluate your strain’s compatibility with the engineering process.

Perform an Onyx genome engineering run using the Onyx Strain Assessment Kit.

Assess editing performance in your strain using Inscripta’s Genotyping Assays.