Accelerate innovation with Iterative Genome Engineering


Iterative Genome Engineering – our latest feature to accelerate your research

Our protocols and kits provide a convenient, streamlined, and validated way to repeatedly introduce additional edits into cells to recombine genetic variants and accelerate strain engineering. Iterative Genome Engineering with the Onyx® platform facilitates fast and easy stacking of edits and allows you to:

  • explore interactions between genomic variants
  • discover synergistic effects and unlock new biological mechanisms
  • rapidly improve production strains by stacking beneficial edits.

Watch the video to see how our customer Sestina Bio is using Iterative Genome Engineering in their projects. 

What’s really exciting is […] using what we’ve learned from the last cycle to guide the library design in the next cycle.” 

See what you can do with Iterative Genome Engineering

Whether you are working on engineering a more efficient enzyme or trying to improve productivity of your strain, the process requires multiple genome editing cycles. Watch the webinar to see how you can rapidly achieve significant improvement in protein production and activity with the Onyx Iterative Genome Engineering workflow. 

Eric Abbate — Director of Analytical Biochemistry, Applications Development, Inscripta

Design. Discover. Repeat

Iterative Genome Engineering allows you rapidly move through the Design-Generate-Test-Learn (DGTL) cycle to accelerate the discovery process. After screening your Onyx Cell Libraries and identifying the variants you want to take forward, you can design new edit libraries to generate additional diversity. To do that, the editing plasmids are cured from the engineered cells, which can be then transformed with new editing reagents. Update and import the new genome in just a few minutes using our software and proceed to design your next Genome Engineering Experiment.
Iterative Editing workflow figure R0 01

Iterative Genome Engineering products

The Iterative Genome Engineering protocols and kits enable easy workflow integration, with step-by-step validated instructions on how to prepare your strains for the next genome engineering cycle. With our workflow, you can get to the next round of editing in less than a week.
E. coli

We offer an Onyx® E. coli Curing Kit enabling instrument curing of up to 24 variants and supporting documents describing the curing and preparation for Iterative Genome Engineering. 

S. cerevisiae

For S. cerevisiae, we provide a validated, manual plasmid curing protocol (up to 24 variants), as well as protocols describing the preparation of strains for Iterative Genome Engineering.