MAD7 in mammalian cells
Royalty-free CRISPR nuclease for all R&D applications
MAD7 activity in mammalian cells
While MAD7 has shown robust editing activities in both prokaryotic and eukaryotic microbes, it has also been shown to have DNA editing activity in mammalian cells.
Here we show that MAD7 is able to be expressed as an active protein in human HEK293T cells, and when combined with chemically synthesized guide RNAs targeting multiple genes, can edit several genes at multiple loci.
This precision editing ability, coupled with the ability to use MAD7 royalty-free for commercial R&D purposes, makes MAD7 an ideal tool for precision editing workflows.
MADzymes are a family of novel CRISPR nucleases that have improved features such as different PAM recognition sequences, different cut efficiencies, reduced sizes, and different enzyme kinetics than other CRISPR nucleases.
Inscripta strives to promote widespread exploration and adoption of CRISPR tools in both academic and commercial settings, and has released the DNA sequence of a first enzyme, MAD7, that is royalty-free for all R&D use.